The immunomodulatory effect of Glagah consortium microalgae polysaccharides based on splenocyte proliferation and cytokine production in vitro

Abstract. Afililla Z, Suwanti LT, Mufasirin, Koerniawan HP, Suyono EA, Budiman A, Siregar UJ. 2022. The immunomodulatory effect of Glagah consortium microalgae polysaccharides based on splenocyte proliferation and cytokine production in vitro. Biodiversitas 23: 5205-5209. Indonesia is known as a maritime country that has various organisms, including microalgae. Some have many bioactivities, such activity. This study aimed to determine polysaccharide (GCMP) Spirulina platensis (PSP) an immunomodulator its Crude were extracted by lye extraction method. Splenocytes obtained from spleen healthy mice cultured RPMI 1640 medium with without Concanavalin A (Con A). Cells (3×105 per well) treated dose gradually 0, 1.25, 2.5, 10 20 µg/mL. observed 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide (MTT) assay. supernatant was taken for the detection cytokines using enzyme-linked immunoassay (ELISA). results showed GCMP, both Con at concentration less than mg/mL decreased cell viability, other hand, 10-20 had proliferative effect. shown all concentrations PSP, A. polysaccharides, GCMP did not affect levels. In conclusion, splenocytes, depending concentration.